Journal: Journal of Experimental Botany

Article Title: Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum

doi: 10.1093/jxb/eraa304

Figure Lengend Snippet: ChiVMV HCPro directly interacts with CAT1 and CAT3 in vitro and in vivo . (A) Yeast two-hybrid assay. The ability of cells to grow on synthetic dropout medium lacking Leu, Trp, His, and Ade (-LWHA) suggested the interaction. (B) GST pull-down assay showing the interaction among HCPro, CAT1, and CAT3 in vitro . Purified CAT1–MBP, CAT2–MBP, CAT3–MBP, or MBP was incubated with HCPro–GST. After being immunoprecipitated with GST beads, the proteins were detected by protein gel blot analysis with anti-MBP or anti-GST antibodies. (C) BiFC assay. HCPro interacted with CAT1 and CAT3 in N. benthamiana leaves.Scale bars=30 μm.

Article Snippet: HCPro , CAT1 , CAT2 , and CAT3 were amplified by PCR and then inserted into the pGEX-glutathione S -transferase (GST) tag and pMAL-C2X maltose-binding protein (MBP) tag.

Techniques: In Vitro, In Vivo, Y2H Assay, Pull Down Assay, Purification, Incubation, Immunoprecipitation, Western Blot, Bimolecular Fluorescence Complementation Assay

Journal: Journal of Experimental Botany

Article Title: Chilli veinal mottle virus HCPro interacts with catalase to facilitate virus infection in Nicotiana tabacum

doi: 10.1093/jxb/eraa304

Figure Lengend Snippet: Identification of HCPro domains responsible for the interaction between host factors and HCPro. (A) Schematic description of deletion mutants of ChiVMV HCPro. HCPro can be divided schematically into three regions: the N-terminus (residues 1–100), central region (residues 101–300), and C-terminus (residues 301–457). (B) Identification of the interaction specificity between CAT1 and HCPro from different sources. (C) The C-terminus of HCPro is necessary for the interaction. Different purified deletion mutants of HCPro–MBP or MBP were incubated with CAT1–GST or CAT3–GST. After being immunoprecipitated with GST beads, the proteins were detected by protein gel blot analysis with anti-MBP or anti-GST antibodies.

Article Snippet: HCPro , CAT1 , CAT2 , and CAT3 were amplified by PCR and then inserted into the pGEX-glutathione S -transferase (GST) tag and pMAL-C2X maltose-binding protein (MBP) tag.

Techniques: Purification, Incubation, Immunoprecipitation, Western Blot